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b16-f10  (ATCC)


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    Structured Review

    ATCC b16-f10
    B16 F10, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7917 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b16+f10+melanoma+cells/B16-F10/custom%40crl-6475%4042612638
    Average 99 stars, based on 7917 article reviews
    b16-f10 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Injection:

    Article Title: Utilizing vaccines to treat cancer and enhance the success rate of cancer immunotherapy
    Article Snippet: .. Tumor Challenge For tumor challenge experiments, B6 and NSG mice were anesthetized with isoflurane and administered 100,000-150,000 B16-F10 melanoma cells (ATCC) via intravenous or intradermal injection and BALB/c mice were anesthetized with isoflurane, administered 100,000-150,000 4T1 triple-negative breast cancer cells (ATCC) in the mammary fat pad or administered 100,000-150,000 CT26 colon carcinoma cells via intradermal injection. .. Cancer cell lines were cultured in DMEM (Gibco), 10% fetal bovine serum (Sigma Aldrich), 100 units/mL penicillin (Gibco), 100 mg/mL streptomycin (Gibco), and 0.29 mg/mL glutamine (Gibco) prior to harvesting for tumor injection.

    Cell Culture:

    Article Title: Ultrasound-assisted size tuning of polyacrylic acid coated magnetic nanoparticle clusters for biomedical applications.
    Article Snippet: The Logworth value is defined as the negative base-10 logarithm of the p-value: Logworth = − log10(p-value) (2) All described statistical calculations, as well as the creation of the DoE, were done using JMP Pro 18.0.2 (2025 JMP® JMP Statistical Discovery LLC, USA). .. B16-F10 melanoma cells (ATCC, USA) were seeded (1.5⋅104/well, 6-well plates) in DMEM (Gibco®, Life Technologies, UK) containing 10 % fetal bovine serum (FBS) (Sigma-Aldrich, Germany) and 1 % GlutaMAXTM (Gibco®, Life Technologies, UK) and cultured at 37 ◦C (5 % CO2). ..

    Article Title: Modified IL-2 proteins, peg conjugates, and uses thereof
    Article Snippet: .. The anti-tumor activity of exemplary modified IL-2 proteins and modified IL-2 protein-PEG conjugates was assessed in B16-F10 melanoma tumor model. B16-F10 melanoma cells were purchased from ATCC and cultured at 37° C., 5% CO2 in high-glucose DMEM (Gibco) containing 10% FBS (Gibco), and 2 mM L-glutamine. ..

    Article Title: Embryonic angiogenesis markers and diagnostic and therapeutic strategies based thereon
    Article Snippet: .. Amount used for vaccination Protein Size (kDa) (μg/mouse) TRX 13.0 20 TRXtr 7.6 10 TRX-Vimentin 67.0 100 TRXtr-Vimentin 61.0 90 Cell Culture T241 fibrosarcoma cells and B16-F10 melanoma cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco's modified eagle medium (DMEM) (cat. no. BE12-604F, Lonza Benelux B.V., Breda, The Netherlands)+2 mM L-glutamine (cat no. 17-605C, Lonza Benelux B.V.)+100 U/ml penicillin/streptomycin (cat. no. DE17-602E Lonza Benelux B.V.) supplemented with 10% Fetal calf serum (FCS) (cat. no. S1810-500, Biowest, Nuaille, France). .. CT26 colon carcinoma cells (American Type Culture Collection) were cultured in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% FCS.

    Article Title: A Bioinformatics-Guided Analytical Approach for Drug Repositioning: Colchicine as a Candidate for Gaucher Disease Treatment.
    Article Snippet: Gaucher disease is a rare lysosomal storage disorder characterized by lipid accumulation.. Emerging evidence suggests that patients with gaucher disease are at an increased risk of developing melanoma, yet the shared molecular mechanisms remain unclear.. This study aimed to investigate and validate potential biomarkers commonly expressed in gaucher disease and melanoma through an integrated approach combining bioinformatics, in silico, and in vitro analyses.

    Activity Assay:

    Article Title: Modified IL-2 proteins, peg conjugates, and uses thereof
    Article Snippet: .. The anti-tumor activity of exemplary modified IL-2 proteins and modified IL-2 protein-PEG conjugates was assessed in B16-F10 melanoma tumor model. B16-F10 melanoma cells were purchased from ATCC and cultured at 37° C., 5% CO2 in high-glucose DMEM (Gibco) containing 10% FBS (Gibco), and 2 mM L-glutamine. ..

    Modification:

    Article Title: Modified IL-2 proteins, peg conjugates, and uses thereof
    Article Snippet: .. The anti-tumor activity of exemplary modified IL-2 proteins and modified IL-2 protein-PEG conjugates was assessed in B16-F10 melanoma tumor model. B16-F10 melanoma cells were purchased from ATCC and cultured at 37° C., 5% CO2 in high-glucose DMEM (Gibco) containing 10% FBS (Gibco), and 2 mM L-glutamine. ..

    Article Title: Intratumoral sotigalimab with pembrolizumab induces rapid activation of antigen presenting cells and drives anti-tumor responses in non-injected tumors in metastatic melanoma: A phase I/II study
    Article Snippet: 1 Title: Intratumoral sotigalimab with pembrolizumab induces rapid activation of antigen presenting cells and drives anti-tumor responses in non-injected tumors in metastatic melanoma: A phase I/II study Authors: Salah-Eddine Bentebibel,1* Daniel J. McGrail,2,11 Veena Kochat,3,4 Emre Arslan,3,4 Reham Abdel-Wahab,1,11,12,13,14 Barbara Pazdrak,1 Ravi Murthy,5 Nourel Hoda M. Tahon,6 SungNam Cho,1 Dzifa Y. Duose,7 Khalida Wani,7 Jingjing Liu,3 Cara Haymaker,7 Javier A Gomez,8 Heather Sonnemann,1 Arjun S. Katailiha,1 Barbara Nassif-Rausseo,1 Maha Rahim,9 Irene Li9, Aaron T. Mayer,9 Xiaodong Yang,10 Frank J. Hsu,10 Jianhua Zhang,3 Daniel H. Johnson,1 Rodabe N. Amaria,1 Isabella C. Glitza Oliva,1 Sapna P. Patel,1 Patrick Hwu,1 Khaled M Elsayes,6 Hussein A. Tawbi,1 Jared K. Burks,8 Chantale Bernatchez,1 Michael A. Davies,1 Kunal Rai,3,4 Gregory Lizée,1 Suhendan Ekmekcioglu,1,11,13 and Adi Diab,1,11,12,13*

    Article Title: Embryonic angiogenesis markers and diagnostic and therapeutic strategies based thereon
    Article Snippet: .. Amount used for vaccination Protein Size (kDa) (μg/mouse) TRX 13.0 20 TRXtr 7.6 10 TRX-Vimentin 67.0 100 TRXtr-Vimentin 61.0 90 Cell Culture T241 fibrosarcoma cells and B16-F10 melanoma cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco's modified eagle medium (DMEM) (cat. no. BE12-604F, Lonza Benelux B.V., Breda, The Netherlands)+2 mM L-glutamine (cat no. 17-605C, Lonza Benelux B.V.)+100 U/ml penicillin/streptomycin (cat. no. DE17-602E Lonza Benelux B.V.) supplemented with 10% Fetal calf serum (FCS) (cat. no. S1810-500, Biowest, Nuaille, France). .. CT26 colon carcinoma cells (American Type Culture Collection) were cultured in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% FCS.



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    │ PD-L1 degradation efficacy in vitro and in vivo by PLT-TACs in <t>B16F10</t> cells. A , Western blot analysis of PD-L1 in IFN-γ pre-treated B16F10 cells after treated with saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h (at an equivalent amount of 5 μM) for 36 h (n = 3 biologically independent samples). B , Mean fluorescence intensity of levels of surface PD-L1 in IFN-γ pre-treated B16F10 after treated with saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h , measured by FCM (at an equivalent amount of 5 μM) for 36 h (n = 3 biologically independent samples). C , Schematic schedule of the treatment and evaluation in C57BL/6 mice with B16F10 homografting tumor model. D , Tumor growth curves, E , tumor weights, F , images of dissected tumors, G , survival curves from treatment groups (saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h ) at a dosage of 5 mg/kg (n = 6 biologically independent samples). H , Immunohistochemical images of PD-L1 in dissected B16F10 tumors after treatment. I , Mean fluorescence intensity of PD-L1 in CD45-negative tumor cells from dissected tumors after treatment (n = 4 biologically independent samples). Data are presented as mean ± SD. p values were determined by one-way ANOVA test. ns, no significance; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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    Image Search Results


    │ PD-L1 degradation efficacy in vitro and in vivo by PLT-TACs in B16F10 cells. A , Western blot analysis of PD-L1 in IFN-γ pre-treated B16F10 cells after treated with saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h (at an equivalent amount of 5 μM) for 36 h (n = 3 biologically independent samples). B , Mean fluorescence intensity of levels of surface PD-L1 in IFN-γ pre-treated B16F10 after treated with saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h , measured by FCM (at an equivalent amount of 5 μM) for 36 h (n = 3 biologically independent samples). C , Schematic schedule of the treatment and evaluation in C57BL/6 mice with B16F10 homografting tumor model. D , Tumor growth curves, E , tumor weights, F , images of dissected tumors, G , survival curves from treatment groups (saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h ) at a dosage of 5 mg/kg (n = 6 biologically independent samples). H , Immunohistochemical images of PD-L1 in dissected B16F10 tumors after treatment. I , Mean fluorescence intensity of PD-L1 in CD45-negative tumor cells from dissected tumors after treatment (n = 4 biologically independent samples). Data are presented as mean ± SD. p values were determined by one-way ANOVA test. ns, no significance; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Bioactive Materials

    Article Title: “Artificial platelet injection system”: a plug-and-play platelet-based lysosome-targeting chimera for targeted protein degradation

    doi: 10.1016/j.bioactmat.2026.01.011

    Figure Lengend Snippet: │ PD-L1 degradation efficacy in vitro and in vivo by PLT-TACs in B16F10 cells. A , Western blot analysis of PD-L1 in IFN-γ pre-treated B16F10 cells after treated with saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h (at an equivalent amount of 5 μM) for 36 h (n = 3 biologically independent samples). B , Mean fluorescence intensity of levels of surface PD-L1 in IFN-γ pre-treated B16F10 after treated with saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h , measured by FCM (at an equivalent amount of 5 μM) for 36 h (n = 3 biologically independent samples). C , Schematic schedule of the treatment and evaluation in C57BL/6 mice with B16F10 homografting tumor model. D , Tumor growth curves, E , tumor weights, F , images of dissected tumors, G , survival curves from treatment groups (saline, PLT-anti-PD-L1, PLT-TAC l , PLT-TAC m or PLT-TAC h ) at a dosage of 5 mg/kg (n = 6 biologically independent samples). H , Immunohistochemical images of PD-L1 in dissected B16F10 tumors after treatment. I , Mean fluorescence intensity of PD-L1 in CD45-negative tumor cells from dissected tumors after treatment (n = 4 biologically independent samples). Data are presented as mean ± SD. p values were determined by one-way ANOVA test. ns, no significance; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: Breast tumor cell line 4T1 and melanoma tumor cell line B16F10 were purchased from the American Type Culture Collection (ATCC), and cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin.

    Techniques: In Vitro, In Vivo, Western Blot, Saline, Fluorescence, Immunohistochemical staining